Composite

Part:BBa_K2128200:Design

Designed by: Team Genspace2016   Group: iGEM16_Genspace   (2016-10-19)


MAHS with strong promoter/RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have attempted to maximize expression of MAHS through use of a strong constitutive promoter (J23100) and strong ribosome binding site (B0034).


Source

MAHS was taken from the genome of Ramazzottius varieornatus and optimized by the IDT Codon Optimization Tool for E. Coli K12.

References